We have investigated the expression and pharmacology of endothelin (ET) receptors in human aortocoronary saphenous vein grafts.Subtype-selective ligands were used to autoradiographically identify ETA ([125I]-PD151242) and ETB ([125I]-BQ3020) receptors. In graft saphenous vein ETA receptors predominated in the media, with few ETB receptors identified. Neither subtype was detected in the thickened neointima.The ratio of medial ETA:ETB receptors was 75% : 25% in both graft and control saphenous vein.ET-1 contracted control (EC50 2.9 nM) and graft (EC50 4.5 nM) saphenous vein more potently than diseased coronary artery (EC50 25.5 nM).In all three blood vessels ET-1 was 100 times more potent than ET-3 and three times more potent than sarafotoxin 6b (S6b). Little or no response was obtained in any vessel with the ETB agonist sarafotoxin 6c (S6c).The ETA antagonist PD156707 (100 nM) blocked ET-1 responses in all three vessels with pKb values of approximately 8.0.For individual graft veins the EC50 value for ET-1 and ‘age' of graft in years showed a significant negative correlation.In conclusion there is no alteration in ET receptor expression in the media of saphenous veins grafted into the coronary circulation compared to control veins. ETA receptors predominantly mediate the vasoconstrictor response to ET-1 in graft vein, with no apparent up-regulation of ETB receptors. The sensitivity of the graft vein to ET-1 increased with graft ‘age', suggesting that these vessels may be particularly vulnerable to the increased plasma ET levels that are detected in patients with cardiovascular disease.
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